New Anti-Müllerian Hormone (AMH) Antibodies

A diagnostic marker for fertility and ovarian reserve

Anti-müllerian hormone (AMH) has gained widespread interest as an indicator of ovarian function and fertility. Serum levels serve as a biomarker for the relative size of a woman’s ovarian reserve and have clinical importance in predicting the success of in vitro fertilization (IVF). AMH can also be used as a surrogate diagnostic marker of polycystic ovary syndrome in cases when ultrasonographic examination is not possible.

The AMH gene encodes an inactive homodimer precursor of identical disulphide-linked 70-kDa glycoproteins divided into two regions: pro-AMH (or N-terminal) and a C-terminal domain (also called the "mature" region). For signaling activation and binding to the AMH Receptor II, these domains require cleavage to yield two separate homodimers that remain associated in a noncovalent complex. Current commercial assays for AMH use antibody pairs that recognize the mature region only, or both the pro-AMH and mature regions.

Meridian Anti-Müllerian Hormone Antibodies

Meridian has development new AMH antibodies produced in chickens using a synthetic peptide representing a linear epitope on the human AMH protein. Initial purification of total IgY from eggs was followed by affinity purification against the peptide as an immunogen to yield a monospecific antibody. All antibodies can be used in ELISA.