| 品牌 | Fitzgerald | 貨號 | 35R-1900 |
| 價格 | ￥0.00 | 規(guī)格 | 100 ul |
| 應用 | WB | 保存/運輸 | |
| 貨期 | 3~5周 | ||
| 類型 | Controls & Calibrators | 宿主/來源 | |
| 說明書 | 點擊查看 | 訂購量 |
|
組成:Cells were cultured for 30 minutes in the presence of 1mM H2O2 (simulated?cells only)?and lysed in Lysis buffer (20mM HEPES, pH7.2, 20mM Phosphoglycerate, 150mM NaCl, 10% glycerol, 1% NP-40, 1mM EDTA, 1mM EGTA, 1mM Na3VO4, 5mM NaF, 1mM PMSF, 5ug/ml Pepstatin A, 5ug/ml Aprotinin, 5ug/ml Leupeptin). They?were?then diluted with reducing sample buffer (60mM Tris, pH 6.8, 6% glycerol, 2% SDS, 0.2% bromphenolblue, 200mM 2-mercaptoethanol).
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